Robustness of holographic video microscopy against defects in illumination

We numerically investigate how defects in the illumination system of a holographic microscope, in particular divergence and tilt of the illuminating laser beam, affect the measured properties of colloidal spherical particles. We show that under typical experimental conditions divergence and tilt of the laser beam do not affect the measured parameters significantly, confirming the robustness of the technique against such defects. © 2012 Optical Society of America OCIS codes: (090.1760) Computer holography; (180.6900) Three-dimensional microscopy; (290.5850) Scattering, particles References and links 1. J. Sheng, E. Malkiel and J. Katz. “Digital holographic microscope for measuring three-dimensional particle distributions and motions.” Appl. Opt. 45, 3893–3901 (2006). 2. S.-H. Lee and D. G. Grier. “Holographic microscopy of holographically trapped three-dimensional structures.” Opt. Express 15, 1505–1512 (2007). 3. C. F. Bohren and D. R. Huffman. Absorption and Scattering of Light by Small Particles (Wiley Interscience, New York, 1983). 4. S.-H. Lee, Y. Roichman, G.-R. Yi, S.-H. Kim, S.-M. Yang, A. van Blaaderen, P. van Oostrum and D. G. Grier. “Characterizing and tracking single colloidal particles with video holographic microscopy.” Opt. Express 15, 18275–18282 (2007). 5. F. C. Cheong, B. Sun, R. Dreyfus, J. Amato-Grill, K. Xiao, L. Dixon and D. G. Grier. “Flow visualization and flow cytometry with holographic video microscopy.” Opt. Express 17, 13071–13079 (2009). 6. F. C. Cheong, B. J. Krishnatreya and D. G. Grier. “Strategies for three-dimensional particle tracking with holographic video microscopy.” Opt. Express 18, 13563–13573 (2010). 7. H. Shpaisman, B. J. Krishnatreya and D. G. Grier. “Holographic microrefractometer.” Appl. Phys. Lett. 101, 091102 (2012). 8. F. C. Cheong, S. Duarte, S.-H. Lee and D. G. Grier. “Holographic microrheology of polysaccharides from Streptococcus mutans biofilms.” Rheol. Acta 48, 109–115 (2009). 9. L. Dixon, F. C. Cheong and D. G. Grier. “Holographic particle-streak velocimetry.” Opt. Express 19, 4393–4398 (2011). 10. F. C. Cheong, K. Xiao, D. J. Pine and D. G. Grier. “Holographic characterization of individual colloidal spheres’ porosities.” Soft Matter 7, 6816–6819 (2011). 11. J. A. Lock and G. Gouesbet. “Generalized Lorenz-Mie theory and applications.” J. Quant. Spectr. Rad. Trans. 110, 800–807 (2009). 12. G. Gouesbet. “T-matrix fomulationand generalized Lorenz-Mie theories in spherical coordinates.” Opt. Commun. 283, 517–521 (2010). 13. Y. Roichman, B. Sun, A. Stolarski and D. G. Grier. “Influence of non-conservative optical forces on the dynamics of optically trapped colloidal spheres: The fountain of probability.” Phys. Rev. Lett. 101, 128301 (2008). 14. B. Sun, J. Lin, E. Darby, A. Y. Grosberg and D. G. Grier. “Brownian vortexes.” Phys. Rev. E 80, 010401(R) (2009).